Abstract The first description of the fourth instar larva and pupa of Palpomyia mapuche Spinelli, Grogan & Ronderos and the pupa of P. subfuscula Ingram & Macfie are provided, as well as the redescription of the pupa of P. subaspera (Coquillett). Studied specimens were collected in lotic environments of Argentinian Patagonia, in Neuquén and Chubut Provinces. The described stages were examined and illustrated with a phase-contrast microscope. The larva was examined using a scanning electron microscope. Data on the bionomics for P. mapuche and new records for the three species are provided.

Abstract: The fourth instar larva and pupa of Dasyhelea flavifrons Guérin-Méneville are redescribed, illustrated, and photomicrographed using binocular, phase-contrast, and scanning electron microscopy. Comparisons with the American species of the grisea group were made. The immatures were collected by using a siphon bottle in tree-holes and from water collected in dead snail shells in Salta Province, Argentina, transported to the laboratory and there bred to the emergence of the adults. Details on larval habitats are given. These are the first records from Argentina and in gastrotelmata.

ABSTRACT A new monotypic genus and species of predaceous midge from the southernmost area of the Argentinean Yungas, Yungahelea australis Spinelli and Ronderos, is described and illustrated from male and female adults. It belongs to a group containing the Ceratopogonini genera Parabezzia Malloch, Diaphanobezzia Ingram and Macfie, Spinellihelea Borkent, Grogan and Picado, Leptohelea Wirth and Blanton, and Fittkauhelea Wirth and Blanton. Phylogenetic interpretation indicates that Yungahelea is the sister group of Spinellihelea or the clade composed by Parabezzia and Diaphanobezzia.

ABSTRACT The fourth instar larva and pupa of Atrichopogon delpontei Cavalieri and Chiossone are described for the first time. The immatures were collected from stream margins in the northern Brazilian states Rondônia and Piauí, and subsequently reared to adults. Larvae and pupae are illustrated and photomicrographed. Details on the rearing process and feeding behavior in laboratory, bionomics and notes on habitats are also provided.

RESUMO A larva de quarto ínstar de Dasyhelea mediomunda Minaya é descrita pela primeira vez e a descrição completa da pupa é fornecida, através da utilização de microscópio de contraste de fases e microscopia eletrônica de varredura. Os espécimes estudados foram coletados em um "mallin" conectado à zonas úmidas na estepa patagônia, Província de Chubut, Argentina.

ABSTRACT The fourth instar larva of Dasyhelea mediomunda Minaya is described for the first time and a complete description of the pupa is provided, through use of phase-contrast microscope and scanning electron microscope. Studied specimens were collected in a pond connected to a small wetland "mallin" on the Patagonian steppe, Chubut province, Argentina.

The male of Diaphanobezzia patagonica Spinelli & Grogan is described and photographed from specimens collected in association with females in the Somuncura plateau, in the steppe area of central Patagonia. The male herein described is compared with the congeners D. pellucida Ingram & Macfie and D. spinellii Wirth & Grogan. Besides, D. patagonica is recorded from the Laguna Blanca National Park.

Se describe e ilustra el macho de Diaphanobezzia patagonica Spinelli & Grogan sobre la base de ejemplares capturados en asociación con hembras en la meseta de Somuncura, en la estepa de la Patagonia central. El macho aquí descripto es comparado con los machos de los congéneres D. pellucida Ingram & Macfie y D. spinellii Wirth & Grogan. Además, se registra a D. patagonica del Parque Nacional Laguna Blanca.

A new species of predaceous midge, Austrosphaeromias setosa sp. nov., is described and illustrated from adult males and females collected in the Patagonian-Andean region of Argentina and Chile. Based on examination of the type species of Austrosphaeromias Spinelli, 1997 and recently collected specimens from near the type-locality, the female and previously unknown male of Austrosphaeromias chilensis (Ingram & Macfie, 1931) are also described and illustrated. Descriptions are accompanied by color photographs and illustrations of key features of females and males of both species. We also provide a key to adult females and males of the four species of Austrosphaeromias.

Se describe e ilustra una nueva especie de mosquita predadora, Austrosphaeromias setosa sp. nov., a partir de adultos macho y hembra colectados en la región Andino-patagónica de Argentina y Chile. Además, sobre la base del examen de la especie tipo de Austrosphaeromias Spinelli, 1997 y de ejemplares recientemente colectados en las proximidades de la localidad tipo, se describen e ilustran la hembra de Austrosphaeromias chilensis (Ingram & Macfie, 1931) y el macho, previamente desconocido. Las descripciones son acompañadas por fotografías a color e ilustraciones de los caracteres clave de hembras y machos de ambas especies. Se brinda, además, una clave para adultos hembra y macho de las cuatro especies de Austrosphaeromias.

The adult and pupa of a new species of Forcipomyia (Phytohelea) are described, illustrated and photographed. Pupae were collected from water held by Eryngium L. sp. (Apiaceae) in Corrientes province, Argentina and reared to adults in the laboratory. Adult and pupa are compared with the closely related congener F. (P.) jocosa Saunders, and the pupa is also compared with that of F. (P.) bromelicola (Lutz).

Se describen, ilustran y fotografían el adulto y la pupa de una nueva especie de Forcipomyia (Phytohelea). Las pupas fueron capturadas del agua acumulada por Eryngium L. sp. (Apiaceae) en la provincia de Corrientes, Argentina y criadas en el laboratorio hasta la emergencia del adulto. Se compara esta especie con su congénere más cercano, F. (P.) jocosa Saunders, y además, la pupa es comparada con la de F. (P.) bromelicola (Lutz).

The pupal exuvia of Mallochohelea termophila (Spinelli) is redescribed, photographed and illustrated mainly based on the type-series. Pupae of the type-series were collected with forceps from a thermal stream and were attached to rocks about 200 m from the spring head. Pupae from Uruguay were collected with a dipper in lotic and lentic environments. They were reared to adults in the laboratory. This is the first detailed pupal description of a species of Mallochohelea, and the first record of this species from Uruguay. Similarities and differences with the pupae of Neobezzia are provided.

Se describe, fotografía e ilustra la exuvia pupal de Mallochohelea termophila (Spinelli), en especial sobre la base de la serie-tipo. Las pupas de la serie-tipo fueron recolectadas mediante pinzas en un arroyo termal, adheridas a rocas, aproximadamente a 200 m del manatial y las pupas de Uruguay mediante el uso de un cucharón tanto en ambientes lóticos como lénticos. Ellas fueron criadas hasta adultos en el laboratorio. Ésta es la primera descripción detallada de una pupa de una especie de Mallochohelea y el primer registro de la especie para Uruguay. Se destacan semejanzas y diferencias con las pupas de Neobezzia.

The pupa of Parabezzia balseiroi Spinelli & Grogan was described and illustrated using binocular microscope, camera lucida and digital photographs. Pupae were captured along a stream margin in the Parque Provincial E. Tornquist Reserve, Sierra de la Ventana, Buenos Aires province, Argentina. Also, additional pupae were observed, measured and photographed on the basis of the type series deposited in the Museo de La Plata. Parabezzia balseiroi is compared with P. alexanderi Wirth, which was also herein studied and photographed from Nearctic specimens.

Se describe e ilustra la pupa de Parabezzia balseiroi Spinelli & Grogan, utilizando microscopio binocular, cámara clara y fotografía digital. Las pupas fueron capturadas en el margen de un arroyo en la reserva Parque Provincial E. Tornquist, Sierra de la Ventana, provincia de Buenos Aires, Argentina. Asimismo, se observaron, midieron y fotografiaron las pupas de la serie tipo depositadas en el Museo de La Plata. Se compara Parabezzia balseiroi con P. alexanderi Wirth, la cual ha sido también estudiada y fotografiada en este trabajo, sobre la base de ejemplares neárticos.

Pupa e adulto macho previamente desconhecidos de Neobezzia fittkaui Wirth & Ratanaworabhan (Diptera, Ceratopogonidae). A pupa de Neobezzia fittkaui Wirth & Ratanaworabhan, 1972 foi coletada em macrófitas aquáticas flutuantes (Salvinia auriculata Aubl., Salviniaceae) na Ilha da Marchantaria próximo a Manaus, Brasil e o adulto macho criado foi descrito, fotografado e ilustrado pela primeira vez. Esta é a primeira descrição para a pupa do gênero Neobezzia Wirth & Ratanaworabhan.

The previously unknown pupa and adult male of Neobezzia fittkaui Wirth & Ratanaworabhan (Diptera, Ceratopogonidae). The pupa of Neobezzia fittkaui Wirth & Ratanaworabhan, 1972, collected from a mat of floating fern (Salvinia auriculata Aubl., Salviniaceae) in Ilha da Marchantaria near Manaus, Brazil and the reared adult male are described, photographed and illustrated for the first time. This is the first detailed pupal description for the genus Neobezzia Wirth & Ratanaworabhan.

Alrededor de 230 especies de ceratopogónidos han sido registradas o descritas en Argentina, 38 de ellas son conocidas para la provincia de Buenos Aires y sólo una ha sido previamente citada para la Isla Martín García. En este trabajo se presentan los resultados obtenidos a partir de muestreos realizados en seis viajes a la isla durante la primavera de 2005, verano de 2006 y otoño de 2009; se seleccionaron diez sitios de muestreo con ambientes acuáticos permanentes y temporales. La mayoría de los muestreos se llevaron a cabo en cuerpos de agua de diverso origen; algunos de éstos se hallaban cubiertos con vegetación flotante compuesta por Lemna gibba, Lemna minuscule, Salvinia biloba, Salvinia minima, Azolla filiculoides, Limnobium laevigatum, Pistia stratiotes, Spirodela intermedia, Wolffiella oblonga y Wolffia columbiana; mientras que los restantes se realizaron en zonas urbanas y suburbanas. Los adultos se capturaron con redes entomológicas a la salida y puesta del sol y con trampas de luz a intervalos de cuatro a cinco horas por la noche, dependiendo de la disponibilidad de electricidad en la isla. Las larvas y pupas fueron recolectadas con diferentes metodologías según las características de los ambientes estudiados; en el agua libre se capturaron con tamices pequeños o pipetas y micropipetas, y en cuerpos de agua con vegetación se utilizó la técnica de flotación para separar los insectos de las hidrófitas flotantes y arraigadas. Se identificaron trece especies de Ceratopogonidae, 3 de Atrichopogon Kieffer, 3 de Forcipomyia Meigen, 2 de Dasyhelea Kieffer, 4 de Culicoides Latreille, y uno de Bezzia Kieffer, todos ellos representan nuevos registros para la isla.

Nearly 230 species of biting midges have been recorded or described from Argentina; 38 of them are known from the Buenos Aires province and only one is cited from Martín García Island. This paper presents the results raised from six collecting trips which took place on the island during spring 2005, summer 2006 and autumn 2009. Diverse sampling sites including permanent and temporary aquatic environments were chosen, most of the ten sampling sites were ponds of diverse origin, some of these environments were covered with floating vegetation as Lemna gibba, Lemna minuscule, Salvinia biloba, Salvinia minima, Azolla filiculoides, Limnobium laevigatum, Pistia stratiotes, Spirodela intermedia, Wolffiella oblonga and Wolffia columbiana. Other sites were placed in urban and suburban areas. Adults were collected with sweep nets at sunrise and sunset and with light traps at intervals of four to five hours at night, depending on electricity availability on the island. Larvae and pupae were collected with different implements depending on characteristics of each surveyed aquatic habitat. In free standing water, they were captured with small sieves or hand pipettes and micropipettes, flotation techniques were utilized for sampling vegetated areas, free and rooted floating hydrophytes were extracted for removing insects among them. Thirteen species of Ceratopogonidae were collected, three of Atrichopogon Kieffer, three of Forcipomyia Meigen, two of Dasyhelea Kieffer, four of Culicoides Latreille, and one of Bezzia Kieffer, all representing new records from the island. Rev. Biol. Trop. 59 (3): 1183-1194. Epub 2011 September 01.

The following five new species of Culicoides from Colombia are described, illustrated and placed to subgenus or species group: Culicoides antioquiensis, Culicoides gabrieli, Culicoides inermis, Culicoides micayensis and Culicoides nigrifemur. C. gabrieli is also known from Peru. When possible, their position in previously published keys is indicated and their features discussed in light of the most recent revisions. A list of 180 Culicoides species known (114) or suspected of being in Colombia (66) is given in a Table. Of these, 12 including the new species are recorded from Colombia for the first time.

The male of Culicoides dureti Ronderos & Spinelli is described and illustrated from specimens collected associated with females in the province of Misiones, Argentina, and a brief description of the female is provided. The species is compared with Culicoides barbosai Wirth & Blanton.

Se describe e ilustra el macho de Culicoides dureti Ronderos & Spinelli y se brinda una breve descripción de la hembra sobre la base de ejemplares capturados, asociados con hembras en la Provincia de Misiones, Argentina. La especie es comparada con Culicoides barbosai Wirth & Blanton.